abstract：:The effect of ageing on thymocyte progenitors in the bone marrow was studied in an in vitro experimental model that permits T lymphocyte development. The model is based on co-culture of BM cells from young and old mice with lymphoid depleted fetal thymus explants. We applied different strategies of thymic colonization...

journal_title：Immunology letters

authors： Globerson A

更新日期：1994-06-01 00:00:00

abstract：:Clone C19 derived from a rat bladder cancer cell line BC31ad constitutively released cytotoxic factor RCF. The serum-free C19-conditioned medium was partially purified by DEAE-Sephacel, Sephacryl S-200 and MonoQ-FPLC chromatographies to the specific activity of 6.4 x 10(6) U/mg protein (LM assay). RCF was a 17-kDa pro...

journal_title：Immunology letters

authors： Hojo H,Kaneko A,Nanya K,Kayagaki N,Hashimoto Y

更新日期：1994-06-01 00:00:00

abstract：:The earliest T-cell precursor population in the adult mouse thymus (low CD4 precursors) may be divided into 85% of cells expressing surface Mel-14 (LECAM-1, the lymphocyte homing receptor) and 15% of cells which are Mel-14. To date, this is the only surface marker for which we have found this population to be heteroge...

journal_title：Immunology letters

authors： Wu L,D'Amico A

更新日期：1994-05-01 00:00:00

abstract：:We have studied the autocrine regulation of essential expression of the intercellular adhesion molecule-1 (ICAM-1) on 8 transitional cell carcinoma (TCC) cell lines (histopathological grades 1-3). The constitutive expression of ICAM-1 was regulated by soluble factors in an autocrine fashion. These factors were produce...

journal_title：Immunology letters

authors： Alexandroff AB,Jackson AM,Esuvaranathan K,Prescott S,James K

更新日期：1994-05-01 00:00:00

abstract：:The tryptophan fluorescence properties of two different peptide complexes of the single-chain H-2Kd (SC-Kd) were studied by means of the single-photon counting technique. The latter enables time-resolved measurements of fluorescence intensity and anisotropy decay parameters relevant to structural and dynamic propertie...

journal_title：Immunology letters

authors： Dittes K,Gakamsky DM,Haran G,Haas E,Ojcius DM,Kourilsky P,Pecht I

更新日期：1994-05-01 00:00:00

abstract：:To study the interactions between Chlamydia trachomatis (CT) and human immunodeficiency virus (HIV) infections, we examined CT serologies in sequential serum samples of male homosexuals (MHS), followed over a mean period of 4 years. Of the MHS studied, 77 were HIV(-), 18 were HIV(+) and 10 patients seroconverted durin...

journal_title：Immunology letters

authors： Schattner A,Hanuka N,Sarov B,Bentwich Z

更新日期：1994-04-01 00:00:00

abstract：:The leukocyte common antigen, CD45, is one of the major glycoproteins on cells of hemopoietic origin showing considerable heterogeneity in both structure and expression. Biochemical heterogeneity has been attributed to differences in the primary sequence and glycosylation. In this paper we report an additional basis f...

journal_title：Immunology letters

authors： Oravecz T,Monostori E,Adrian O,Kurucz E,Andó I

更新日期：1994-04-01 00:00:00

abstract：:To study the mechanism(s) responsible for the appearance of Epstein-Barr virus (EBV)-induced anti-histone autoantibodies, peripheral blood B lymphocytes from healthy donors were infected with EBV and the resulting lymphoblastoid cell lines were tested for secretion of antibodies reacting with histones. It was found th...

journal_title：Immunology letters

authors： Garzelli C,Incaprera M,Bazzichi A,Manunta M,Rognini F,Falcone G

更新日期：1994-03-01 00:00:00

abstract：:The wide distribution of Borrelia burgdorferi, the spirochete causing Lyme borreliosis, represents a human health hazard in many areas of the world. Vaccination has been proposed as an effective prevention strategy. Vaccination experiments were conducted with preparations of recombinant outer surface protein A (OspA) ...

journal_title：Immunology letters

authors： Gern L,Rais O,Capiau C,Hauser P,Lobet Y,Simoen E,Voet P,Pêtre J

更新日期：1994-03-01 00:00:00

abstract：:This paper describes a new murine monoclonal antibody, UN5, raised against human thymocytes. This antibody recognizes a molecule of approximately 45 kDa on thymocytes. Flow cytometric analysis reveals a high intensity of labeling with the majority of thymocytes, whereas only CD20+ cells from peripheral whole-blood sam...

journal_title：Immunology letters

authors： Tassone P,Bonelli P,Tuccillo F,Bond HM,Turco MC,Morrone G,Cecco L,Cerra M,Venuta S

更新日期：1994-02-01 00:00:00

abstract：:A direct correlation between the constitutive expression of IL-1 alpha and reduced tumorigenicity of fibrosarcomas was observed. This was established in fibrosarcoma cell lines which produce IL-1 alpha 'spontaneously', possibly as an aberration of oncogene-mediated transformation or upon IL-1 alpha gene transfer. In f...

journal_title：Immunology letters

authors： Apte RN,Douvdevani A,Zoller M,White RM,Dvorkin T,Shimoni N,Fima E,Hacham M,Huleihel M,Benharroch D

更新日期：1993-12-01 00:00:00

abstract：:Humans frequently produce serum IgM autoantibodies reactive with T-cell receptor beta chains at a determinant defined by peptides corresponding to the first complementarity determining region. It is likely that this determinant serves as a public idiotype involved in immunoregulation. Following screening of culture su...

journal_title：Immunology letters

authors： Dedeoglu F,Kaymaz H,Klein G,Marchalonis JJ

更新日期：1993-11-01 00:00:00

abstract：:Adrenaline has been shown to inhibit the release of tumor necrosis factor (TNF) elicited by lipopolysaccharide (LPS) when tested in vitro on cultured human blood cells and rat macrophages. In this report we have examined the effect of the in vivo administration of adrenaline on TNF serum levels induced by LPS. In agre...

journal_title：Immunology letters

authors： Monastra G,Secchi EF

更新日期：1993-10-01 00:00:00

abstract：:The influence of human recombinant granulocyte-macrophage colony-stimulating factor (rH GM-CSF) on respiratory burst response of isolated human neutrophils was examined. Preincubation of cells with rH GM-CSF significantly increased the respiratory burst in response to formyl-methionyl-leucyl-phenylalanine (FMLP), meas...

journal_title：Immunology letters

authors： Leino L,Nuutila J,Pelliniemi TT,Rajamäki A

更新日期：1993-09-01 00:00:00

abstract：:Pentoxifylline (PTX), a methylxanthine derivative and phosphodiesterase inhibitor, is known to influence production and/or function of some cytokines. We examined the effect of PTX on the in vitro expression of cytokine genes using endotoxin- or phytohaemagglutinin (PHA)-stimulated human blood mononuclear cells. The e...

journal_title：Immunology letters

authors： Rieneck K,Diamant M,Haahr PM,Schönharting M,Bendtzen K

更新日期：1993-08-01 00:00:00

abstract：:Antigen receptor (AgR) crosslinking by antigens or AgR-specific antibodies induces a cascade of enzymatic events in lymphocytes which involves activation of several non-receptor tyrosine- and serine/threonine kinases, phosphatases, phospholipases, etc. Here we show data demonstrating that a thiol group-reactive protei...

journal_title：Immunology letters

authors： Rozsnyay Z,Sarmay G,Gergely J

更新日期：1993-08-01 00:00:00

abstract：:Control animals and rats infected 90 days earlier, by inoculation of 1 x 10(6) trypomastigotes of Trypanosoma cruzi at weaning, were subjected to adult thymectomy (ATx) or sham operation (S-ATx) and assessed 3 months later for the presence of myocardial lesions and levels of lymph node and spleen T-cell populations. C...

journal_title：Immunology letters

authors： Bottasso OA,Revelli SS,Dávila H,Valenti JL,Musso OC,Ferro ME,Romero-Piffiguer M,Morini JC

更新日期：1993-08-01 00:00:00

abstract：:African swine fever (ASF) virus has been reported to infect cells of the monocyte family, probably macrophage-like cells, but there is variation in the apparent susceptibility of these cells. We have demonstrated that the phenotype and activity of porcine monocytic cells varies between different isolations and also up...

journal_title：Immunology letters

authors： McCullough KC,Schaffner R,Fraefel W,Kihm U

更新日期：1993-07-01 00:00:00

abstract：:To test whether apoptotic thymocytes can be identified by altered packing of lipids in their plasma membranes, thymocytes were isolated from mice injected with hydrocortisone and stained with merocyanine 540 (MC540), a fluorescent probe sensitive to lipid packing. At 9-10 h after injection, a subpopulation of H2-Klo c...

journal_title：Immunology letters

authors： Schlegel RA,Stevens M,Lumley-Sapanski K,Williamson P

更新日期：1993-06-01 00:00:00

abstract：:We have investigated the cellular responses of guinea-pig macrophages to human C4a. C4a induced a biphasic Ca2+ mobilization; a rapid temporary Ca2+ mobilization from intracellular Ca2+ pool followed by a weak Ca2+ influx of extracellular Ca2+. Although the C4a-treated macrophages did not respond again to C4a, that is...

journal_title：Immunology letters

authors： Murakami Y,Yamamoto T,Imamichi T,Nagasawa S

更新日期：1993-06-01 00:00:00

abstract：:Parenteral administration of antigen induces in the earthworms Lumbricus terrestris and Eisenia foetida the formation of antigen-binding protein. Labelled antigen was efficiently digested in the coelomic fluids of both species since the total amount of radioactivity in the coelomic fluids decreased. Antigen internaliz...

journal_title：Immunology letters

authors： Rejnek J,Tucková L,Síma P,Bilej M

更新日期：1993-05-01 00:00:00

abstract：:The immunomodulating properties of a neuropeptide hormone, corticotropin-releasing factor (CRF), led us to investigate its effect on cAMP production by human peripheral blood mononuclear cells (MNC). In response to stimulation with CRF (100 nM), a statistically significant (P = 0.019) increase occurred in the amount o...

journal_title：Immunology letters

authors： Singh VK,Leu SJ

更新日期：1993-03-01 00:00:00

abstract：:While the spleen is an active site for myelopoiesis during the late embryonal and perinatal stages, the activity is gradually lost later. However, myelopoiesis in the adult spleen can be reactivated by irradiation or various stimulants. In this study we investigated factors which determine the myelopoiesis-supporting ...

journal_title：Immunology letters

authors： Ohno H,Ogawa M,Nishikawa S,Hayashi S,Kunisada T,Nishikawa S

更新日期：1993-02-01 00:00:00

abstract：:Coelomic fluid from the earthworm Lumbricus terrestris is known to contain immunoactive cells and molecules involved in immune defense. To assess the ability of such molecules to form precipitating complexes with anti-human immunoglobulins, radial immunodiffusion experiments were conducted. Anti-human IgG, anti-human ...

journal_title：Immunology letters

authors： Eyambe GS

更新日期：1993-02-01 00:00:00

abstract：:The production of interleukin 1 (IL-1) by lipopolysaccharide (LPS)-stimulated myelomonocytic cell lines ML-1, THP-1 and PL-21 was significantly enhanced by the addition of insulin, insulin-like growth factor (IGF)-I or IGF-II into the cell cultures. The IL-1 activity in the supernatants from cell cultures stimulated w...

journal_title：Immunology letters

authors： Holán V,Minowada J

更新日期：1992-12-01 00:00:00

abstract：:Antibodies reactive with native double stranded DNA are characteristic of the chronic inflammatory disease systemic lupus erythematosus. Native DNA is however, a poor immunogen and the mechanism of anti-DNA antibody production is incompletely understood. Modification of DNA can increase its immunogenicity and in infla...

journal_title：Immunology letters

authors： Blount S,Griffiths HR,Staines NA,Lunec J

更新日期：1992-10-01 00:00:00

abstract：:Previous studies have demonstrated that culture supernatants of LPS-stimulated murine splenic B lymphocytes (BLPSSN) are able to inhibit the growth of freshly isolated B cells via an IgM antibody. In this work we investigated the progress of LPS-activated B lymphocytes through the cell cycle in the presence of this an...

journal_title：Immunology letters

authors： Uher F,Mihalik R,Alonso ME,Gergely J

更新日期：1992-08-01 00:00:00

abstract：:A375 human melanoma cell cultures grown in the presence of TGF beta contained greatly reduced cell numbers and exhibited drastic alterations in cell morphology compared to the control cultures. Preincubation of the cells with the cytokine for only 18 h was sufficient to induce these changes irreversibly. Examination o...

journal_title：Immunology letters

authors： Larisch-Bloch S,Yarom R,Sulitzeanu D

更新日期：1992-07-01 00:00:00

abstract：:Histamine and putrescine (a precursor of polyamines) are formed by histidine decarboxylase (HDC) and ornithine decarboxylase (ODC), respectively. Within a few hours after injection of a lipopolysaccharide (LPS) into mice, HDC is induced in the liver, spleen, lung and bone marrow, and ODC is induced in the liver, splee...

journal_title：Immunology letters

authors： Endo Y,Kikuchi T,Takeda Y,Nitta Y,Rikiishi H,Kumagai K

更新日期：1992-06-01 00:00:00

abstract：:The protozoan parasite Trypanosoma cruzi is able to replicate in the cytoplasm of primary resident macrophages, but is killed by activated macrophages. Pretreatment of human macrophages with recombinant IFN-gamma and to a lesser extent with TNF-alpha, induced a significant trypanocidal activity. Furthermore, TNF-alpha...

journal_title：Immunology letters

authors： Muñoz-Fernández MA,Fernández MA,Fresno M

更新日期：1992-06-01 00:00:00

abstract：:Mouse trisomy 16 (Ts16), an animal model for human Down syndrome (trisomy 21), exhibits severe abnormalities in the development of lymphoid and myeloid cells. Whereas fetal liver cells from diploid mice can be easily immortalized by retroviral transformation with Ab1-MuLV or J2 virus, fetal livers from Ts16 mice conta...

journal_title：Immunology letters

authors： Berger CN,Epstein CJ

更新日期：1992-06-01 00:00:00

abstract：:Several recent studies have shown that the antigen receptors (mIgM and mIgD) on normal B cell surfaces are part of larger multimolecular complexes, as each is noncovalently coupled to at least two other proteins whose phosphorylation is inducible upon G protein activation. However, B cell receptor complexes appear to ...

journal_title：Immunology letters

authors： Chang CC,Alhasan S,Rosenspire AJ

更新日期：1992-05-01 00:00:00

abstract：:We have explored the requirements for the induction of the N-formyl-methionyl-leucyl-phenylalanine (FMLP) response in Daudi cells after anti-immunoglobulin treatment. Our results indicate that (a) induction of responsiveness to FMLP was observed in Daudi only after crosslinking of surface immunoglobulin by anti-immuno...

journal_title：Immunology letters

authors： Kalunta CI,Kaptein JS,Scott SJ,Lin CK,Gu DG,Lad PM

更新日期：1992-04-01 00:00:00

abstract：:Six hybridomas secreting monoclonal antibodies directed against human fetal liver metallothioneins (MTs) have been generated and characterized. One antibody, K5A6, was specific for MT-1, the others recognized all isoMTs present in the human fetal liver. Each of these antibodies showed a unique cross-reactivity pattern...

journal_title：Immunology letters

authors： Van Houdt K,Nicasi I,Van Mechelen E,Veulemans B,De Ley M

更新日期：1992-03-01 00:00:00

abstract：:Two chicken monoclonal antibodies (MAbs), HU/Ch2-7 and HU/Ch6-1, against heterophil Hanganutziu-Deicher (HD) antigens with N-glycolylneuraminic acid (NeuGc) at a terminal carbohydrate were established by cell fusions using chicken B cell lines lacking thymidine kinase and spleen cells from chickens immunized with II3N...

journal_title：Immunology letters

authors： Asaoka H,Nishinaka S,Wakamiya N,Matsuda H,Murata M

更新日期：1992-03-01 00:00:00

abstract：:A docile substrain of lymphocytic choriomeningitis virus (LCMV) causes a persistent infection in adult C3HeB mice and induces a severe autoimmune hemolytic anemia (AIHA) which is maximal around three weeks post infection (PI). Evaluations of serum immunoglobulin levels of these mice demonstrated grossly elevated IgG2a...

journal_title：Immunology letters

authors： Stellrecht KA,Vella AT

更新日期：1992-02-15 00:00:00

abstract：:IL-6 mediates its pleiotropic functions through two membrane proteins, a ligand-binding molecule (IL-6 receptor, IL-6R) and a non-ligand-binding signal transducer (gp130). Starting with a previously isolated cDNA clone encoding human gp130, recombinant soluble gp130 (sgp130) lacking the transmembrane and cytoplasmic r...

journal_title：Immunology letters

authors： Yasukawa K,Futatsugi K,Saito T,Yawata H,Narazaki M,Suzuki H,Taga T,Kishimoto T

更新日期：1992-02-01 00:00:00

abstract：:Recombinant and pure "natural" IL-1 and IL-2 were compared with the muramyl dipeptide (MDP) component of Freund's adjuvant for their capacity to enhance the humoral immune response against foot-and-mouth disease (FMD) virus antigen. Using a dose of this antigen which alone did not give a detectable immune response, an...

journal_title：Immunology letters

authors： McCullough KC,Pullen L,Parkinson D

更新日期：1992-01-01 00:00:00

abstract：:An automated biosensor system designed for measuring molecular interactions in real time and without any labelling of the reactants has been used to study the interaction of two animal viruses (vaccinia virus and poliovirus) and two plant viruses (cowpea mosaic virus and tobacco mosaic virus) with monoclonal antibodie...

journal_title：Immunology letters

authors： Dubs MC,Altschuh D,Van Regenmortel MH

更新日期：1992-01-01 00:00:00

abstract：:The biological activity of six synthetic peptides of the 124-144 region of the human interferon alpha-2 (IFN alpha-2) molecule was studied. Peptides were examined for their ability to inhibit mitogen induced proliferation of human blood cells in vitro. Only the peptide corresponding to the amino acid sequence 124-138 ...

journal_title：Immunology letters

authors： Danilkovich AV,Freze KV,Shevalier AF,Samukov VV,Kirkin AF,Gusev MV

更新日期：1992-01-01 00:00:00